Title:Characterization of Subdomain IIA Binding Site of Human Serum Albumin in its Native, Unfolded, and Refolded States Using Small Molecular Probes
DOI:10.1021/ja8031289
ligand name:3-Pyridone
ligand smiles:C1=CC(=CN=C1)O
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:site I  

#Binding and thermodynamic parameters#
parameter;
pH;
T;
KSV;2.2011*10^3;
kq;3.45*10^11;
n;1.1
K;
Ka;
Kb;2.3*10^4;
ΔH;
ΔS;
ΔG;
Kd


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;1.36848*10^-15;
E;0.22;
R0;1.81;
r;2.24;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





