Title:Molecular structure-affinity relationship of flavonoids in lotus leaf (Nelumbo nucifera Gaertn.) on binding to human serum albumin and bovine serum albumin by spectroscopic method
DOI:10.3390/molecules22071036
ligand name:Apigenin
ligand smiles:C1=CC(=CC=C1C2=CC(=O)C3=C(C=C(C=C3O2)O)O)O 
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:

#Binding and thermodynamic parameters#
parameter;
pH;
T;300.15;
KSV;2.911*10^6;
kq;4.695*10^14;
n;0.962;
K;
Ka;
Kb;4518559.444;
ΔH;
ΔS;
ΔG;
Kd


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





