Title:Differential scanning calorimetry as a tool to estimate binding parameters in multiligand binding proteins
DOI:10.1016/j.ab.2005.12.029
ligand name:8-anilinonaphthalene-1-sulfonic acid
ligand smiles:O=S(=O)(O)C1=C2C(=CC=C1)C=CC=C2NC1=CC=CC=C1
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:

#Binding and thermodynamic parameters#
parameter;
pH;
T;
KSV;
kq;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#ITC#
parameter;
pH;
T;298;308;318;
n;1;1;1;
K;
Ka;
Kb;0.83*10^6
ΔH;-31.7984;-23.8488;-31.3800;
ΔS;-106.7060;-77.4312;-98.6792;
ΔG;-11.2968;-20.0832;-16.3176;
Cp;-209.2;-209.2;-209.2;
Kd;

#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;

#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;

#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;



#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;

#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





