Title:Unusual Binding of a Potential Biomarker with Human Serum Albumin
DOI:10.1002/asia.201201060
ligand name:BP(OH)2
ligand smiles:C1=CC(=C(N=C1)C2=C(C=CC=N2)O)O 
key residue(hbond):Arg348;Arg-485 d
key residue(hydrophobic):
PDB ID:
Binding Area:Sudlow′s sites I;Sudlow′s sites II 

#Binding and thermodynamic parameters#
parameter;
pH;
T;
KSV;3.8*10^4;
kq;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;-25.9826;
Kd


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





