Title:Fluorescence spectroscopy of osthole binding to human serum albumin
DOI:10.1016/j.jpha.2012.10.002
ligand name:osthole
ligand smiles:CC(=CCC1=C(C=CC2=C1OC(=O)C=C2)OC)C  
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:

#Binding and thermodynamic parameters#
parameter;
pH;
T;298;303;310;
KSV;9.812*10^4;9.666*10^4;9.404*10^4;
kq;
n;0.9958;0.9875;0.9819;
K;
Ka;
Kb;9.298*10^4;8.574*10^4;4.944*10^4;
ΔH;-9.519;-9.519;-9.519;
ΔS;63.041;63.041;63.041;
ΔG;-28.3056;-28.6208;-29.0621;
Kd


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





