Title:Biophysical and theoretical investigation of benzo[c]coumarin functionalized Schiff base with human serum albumin
DOI:10.1007/s11696-020-01496-5
ligand name:1
ligand smiles:COC1=CC=CC(\C=N\C2=CC=C3OC(=O)C4=C(C=CC=C4)C3=C2)=C1O
key residue(hbond):Arg257;Gln196;Glu153;His242;Ser196;Tyr150 d
key residue(hydrophobic):
PDB ID:
Binding Area:site I d

#Binding and thermodynamic parameters#
parameter;
pH;
T;
KSV;4.1*10^3;
kq;4.1*10^11;
n;1;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





