Title:Bromo-Substituted Diazenyl-pyrazolo[1,5-a]pyrimidin-2-amines: Sonogashira Cross-Coupling Reaction, Photophysical Properties, Bio-interaction and HSA Light-Up Sensor
DOI:10.1002/cbic.202200248
ligand name:4c
ligand smiles:CCCCCCC#CC1=CC=C(C=C1)\N=N\C1=C2N=C(C=C(N2N=C1N)C(F)(F)F)C1=CC=CC=C1
key residue(hbond):
key residue(hydrophobic):Trp214;Tyr452;Ala291 d
PDB ID:
Binding Area:

#Binding and thermodynamic parameters#
parameter;
pH;
T;298.15;
KSV;3.74*10^3;
kq;0.66*10^12;
n;1.10;
K;
Ka;2.50*10^3;
Kb;4.04*10^3;
ΔH;
ΔS;
ΔG;
Kd


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





