Title:Far-red pentamethine cyanine dyes as fluorescent probes for the detection of serum albumins
DOI:10.1098/rsos.200453
ligand name:dye 3
ligand smiles:CC[N+]1=C(SC2=C1C1=C(C=CC=C1)C=C2)\C=C1/CC(C)(C)CC(\C=C2/SC3=C(C=CC=C3)N2CCCS([O-])(=O)=O)=C1
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:subdomain IA;sudomain IIA;subdomain IIIA

#Binding and thermodynamic parameters#
parameter;
pH;
T;
KSV;
kq;
n;1;5;
K;
Ka;
Kb;2.9*10^5;3.0*10^4;
ΔH;
ΔS;
ΔG;
Kd;


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





