Title:Human serum albumin unfolding pathway upon drug binding: A thermodynamic and spectroscopic description
DOI:10.1016/j.jct.2008.11.011
ligand name:Promazine
ligand smiles:CN(C)CCCN1C2=CC=CC=C2SC3=CC=CC=C31  
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:

#Binding and thermodynamic parameters#
parameter;
pH;
T;293;
KSV;
kq;1.8*10^12;
n;1.1
K;
Ka;
Kb;3.3*10^4;
ΔH;
ΔS;
ΔG;
Kd


#ITC#
parameter;
pH;
T;293;
n;1.8
K;
Ka;
Kb;2.6*10^4
ΔH;-6.3;
ΔS;64.5051;
ΔG;-25.2
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





