Title:A novel lophine-based fluorescence probe and its binding to human serum albumin
DOI:10.1016/j.aca.2013.04.003
ligand name:DAPIM
ligand smiles:CN(C)C1=CC=C(C=C1)C2=C(N=C(N2)C3=CC=C(C=C3)C(=O)OC)C4=CC=CC=C4
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area: site II(subdomain IIIA)

#Binding and thermodynamic parameters#
parameter;
pH;
T;
KSV;
kq;
n;1.07;
K;
Ka;
Kb;3.65*10^6;
ΔH;
ΔS;
ΔG;
Kd


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





