Title:Comparative studies of unfolding and binding of ligands to human serum albumin in the presence of fatty acid: Spectroscopic approach
DOI:10.1016/j.ijbiomac.2008.03.004
ligand name:Diazepam
ligand smiles:CN1C(=O)CN=C(C2=C1C=CC(=C2)Cl)C3=CC=CC=C3
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:subdomain III

#Binding and thermodynamic parameters#
parameter;
pH;
T;298;
KSV;
kq;
n;0.88;
K;
Ka;
Kb;0.016*10^7;
ΔH;
ΔS;
ΔG;-29.6167;
Kd


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;




#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





