Title:Study on the binding interaction of levofloxacin with human serum albumin in the presence of Ca2+ by fluorescence spectrophotometry
DOI:None
ligand name:levofloxacin
ligand smiles:C[C@H]1COC2=C3N1C=C(C(=O)C3=CC(=C2N4CCN(CC4)C)F)C(=O)O
key residue(hbond):
key residue(hydrophobic):
PDB ID:
Binding Area:

#Binding and thermodynamic parameters#
parameter;
pH;
T;298;310;320;
KSV;43960;41180;40460;
kq;43960*10^8;41180*10^8;40460*10^8;
n;1.1508;1.1171;1.0454;
K;
Ka;2.15*10^5;1.46*10^5;0.690*10^5;
Kb;2.16*10^5;1.46*10^5;0.694*10^5;
ΔH;2.62;2.62;2.62;
ΔS;103.5;103.5;103.5;
ΔG;-30.4;-29.5;-27.6;
Kd;


#ITC#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Cp;
Kd;


#UV-vis absorption spectroscopy#
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#other_method#
method;
parameter;
pH;
T;
n;
K;
Ka;
Kb;
ΔH;
ΔS;
ΔG;
Kd;


#Kinetic Parameters Describing Michaelis–Menten Constant#
HSA/Ligand;RA (%);Vmax;Km;kcat;kcat/Km;



#FRET from Steady State Measurements#
parameter;
pH;
T;
J;
E;
R0;
r;
EFRET;
F0;
F;


#Changes in the ASA(Å2) Values of the Interacting Residues of HSA and ligand Complex
residues;ASA(Å2) of HSA;ASA(Å2)of com;ΔASA(Å2)





